Stereology makes it possible to count the number of objects in a three-dimensional structure from a two-dimentional section.
Based on a set of mathematical and statistical principles, stereology is used to obtain quantitative information through a three-dimensional interpretation of a material from measurements on 2-dimensional sections or scanned images of for example brains, bones and lungs.
Stereology gives you a robust and unbiased result that you can count on!
Avoid methodological errors in your results by using Systematic Uniform Random Sampling (SURS) and the disector-principle. When applying these methods, the objects in a structure have an equal probability of being counted regardless of size, shape and orientation.
By applying unbiased stereology using the newCAST software system from Visiopharm we are able to quantify:
- particles in thin and thick sections (physical and optical disectors),
- the volume and area of any object (cavalieri’s estimator),
- the volume and surface area of particles (optical rotator),
- the surface and length of particles and objects (probes).
Please contact us to find out more about how we can help you with your specific project!
Watch this video article explaining how stereology is used to quantify newly-formed cells in the rat hippocampus following electro convulsive stimulation.